SPAG1 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-15858
宿主: Rabbit
反应性: H
应用: WB,IHC
Overview
| Synonyms | CILD28,FLJ32920,HSD-3.8,Infertility related sperm protein Spag 1,SP75,SPAG1,Sperm associated antigen 1,Tetratricopeptide repeat-containing protein,TPIS,TPR containing protein involved in spermatogenesis,SPAG1抗体 |
| Swissprot | Q07617 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | Synthetic peptide of human SPAG1 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000; |
| Concentration | 0.3 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasmic |
| Tissue specificity | Present in most tissues, including lung, with the strongest expression in brain, colon, kidney, and testis. In sperm and testis, detected in particular in pachytene primary spermatocytes. Up-regulated in pancreatic tumor tissues and not in normal pancreatic tissue. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. |
Images
Western Blot analysis of Human colon tissue using SPAG1 Polyclonal Antibody at dilution of 1:600
Immunohistochemistry of paraffin-embedded Human ovarian cancer using SPAG1 Polyclonal Antibody at dilution of 1:25
Immunohistochemistry of paraffin-embedded Human lung cancer using SPAG1 Polyclonal Antibody at dilution of 1:25
