VCAM-1/CD106 Polyclonal Antibody(Capture/Detector)
| 规格： | / 25μg / 100μg |
| 价格： | / ￥800 / ￥2100 |
货号：AN000250P
货期：咨询
宿主: Rabbit
反应性: H;M;R
应用: WB;IHC;ELISA Capture/Detector
Overview
| Synonyms | CD106;Vcam1;Vcam-1;V-CAM 1;VascuLar ceLL adhesion protein 1;SoLubLe VascuLar CeLL Adhesion MoLecuLe-1 |
| Swissprot | P29534 |
| Source | Rabbit |
| Reactivity | Human;Mouse;Rat |
| Immunogen | Recombinant Rat VCAM-1/CD106 protein expressed by Mammalian |
| Application | WB(Detection kit: E-IR-R304);IHC(Detection kit: E-IR-R213);ELISA Capture/Detector |
| Recommended dilution | WB,,1:500-1:1000;IHC,,1:1000-1:2000;ELISA Capture,,2-8 μg/mL;ELISA Detector,,0.1-0.4 μg/mL |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at 4°C Valid for 1 month or -20°C Valid for 12 months,Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Storage buffer | Sterile PBS,pH 7.4 |
| Background | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses,binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation. |
Images
Western Blot with VCAM-1/CD106 Polyclonal Antibody at dilution of 1:500.Lane 1:Mouse spleen,Lane 2:Rat kidney,Lane 3:Rat spleen.
Immunohistochemistry of paraffin-embedded Human Kidney cancer using VCAM-1/CD106 Polyclonal Antibody at dilution of 1:2000.
Sandwich ELISA-Recombinant Rat VCAM-1/CD106 protein standard curve.Background subtracted standard curve using VCAM-1/CD106 antibody(AN000250P)(Capture),VCAM-1/CD106 antibody(AN000250P)(Detector) in sandwich ELISA.The reference range value for Recombinant Rat VCAM-1/CD106 protein is 31.25-2000 pg/mL.
