XRCC4 Monoclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥580 / ￥1420 / ￥2310 |
货号：E-AB-22044
宿主: Mouse
反应性: H
应用: WB,IHC-p,IF,IP
Overview
| Synonyms | DNA double strand break repair and V(D)J recombination protein XRCC4,DNA repair protein XRCC4,SSMED,X ray repair complementing defective repair in Chinese hamster cells 4,X ray repair cross complementing 4,X ray repair cross complementing protein 4,X-ray repair cross-complementing protein 4,XRCC 4,XRCC4,XRCC4,XRCC4抗体 |
| Swissprot | Q13426 |
| Source | Mouse |
| Reactivity | Human |
| Immunogen | Synthetic Peptide |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF,IP |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:50-300;IF,,1:50-1:200;IP,,1:100-1:300; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Nucleus. |
| Tissue specificity | Widely expressed. |
| Clone No | Clone:8K2 |
|---|---|
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.4 |
| Background | Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. |
Images
Western Blot analysis of 1) Hela, 2) 293T cells using XRCC4 Monoclonal Antibody at dilution of 1:3000.
Immunohistochemistry of paraffin-embedded Human breast cancer tissue using XRCC4 Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Human liver cancer tissue using XRCC4 Monoclonal Antibody at dilution of 1:200.
